Fraunhofer IZI-BB has directed its main focus within the development of bacterial detection systems to the speed of analysis, while also ensuring their use is as straightforward as possible. In general, a method is considered straightforward and fast when established techniques allow gram-positive and/or gram-negative bacteria to be distinguished in just a few minutes without the usual setup of equipment arrays.
Many on-site users of this method (veterinarians, producers of animal foodstuffs, companies processing foodstuffs) have been calling for this for some time, and this method contributes to a more differentiated use of antibiotics.
Our rapid test is based on a range of refractive indices of differing densities as are produced by the use of different matrices in a refractometer. The principle is comparable with the analysis of sugar content, as is performed by winemakers using a hand-held refractometer when determining density using the Oechsle scale.
Another method describes specific, straightforward and rapid detection of bacterial contamination of fluids. By employing a new method established at the Fraunhofer IZI-BB which is used to obtain antigens from bacterial mRNA, monoclonal antibodies are produced that are reduced to Fab fragments and coupled with an electron transfer resonance element that can be detected optically. A specific analysis architecture based on nanobeads allows a visible color signal to be produced when the corresponding bacterial pathogens are present, and which specifically highlights the germs.