Rapid Detection System for Bacteria

Fraunhofer IZI-BB has directed its main focus within the development of bacterial detection systems to the speed of analysis, while also ensuring their use is as straightforward as possible. In general, a method is considered straightforward and fast when established techniques allow gram-positive and/or gram-negative bacteria to be distinguished in just a few minutes without the usual setup of equipment arrays.

Many on-site users of this method (veterinarians, producers of animal foodstuffs, companies processing foodstuffs) have been calling for this for some time, and this method contributes to a more differentiated use of antibiotics.

Our rapid test is based on a range of refractive indices of differing densities as are produced by the use of different matrices in a refractometer. The principle is comparable with the analysis of sugar content, as is performed by winemakers using a hand-held refractometer when determining density using the Oechsle scale.

Another method describes specific, straightforward and rapid detection of bacterial contamination of fluids. By employing a new method established at the Fraunhofer IZI-BB which is used to obtain antigens from bacterial mRNA, monoclonal antibodies are produced that are reduced to Fab fragments and coupled with an electron transfer resonance element that can be detected optically. A specific analysis architecture based on nanobeads allows a visible color signal to be produced when the corresponding bacterial pathogens are present, and which specifically highlights the germs.

Development of an Apparatus-Free, Universal Sensor for Direct Detection of Bacteria – Apparatus-Free Bacteria Sensor

The cooperative project describes a specific, simple and fast means of detecting bacterial contamination of water, giving the project high relevance for the master plans pursued by the Berlin-Brandenburg Food Industry and Health Care Clusters in the region, thereby exploiting synergies with these two clusters. By employing a new method established at the Fraunhofer IZI-BB which is used to obtain antigens from bacterial mRNA, monoclonal antibodies are produced that are reduced to Fab fragments and coupled with an electron transfer resonance element that can be detected optically. A specific analysis architecture based on nanobeads allows a visible color signal to be produced when bacterial pathogens are present, which highlights the specific germs.

Logo-EFRE-Brandenburg

This project is being funded by ERDF.
The Fraunhofer IZI-BB would like to thank the funding authority for the opportunity to realize this research project.

  • Antibody-mediated FRET detection > visual evaluation
  • ATP/NADH-mediated signaling cascades > visual evaluation
  • Dipstick-integrated isothermal amplification > visual evaluation
  • Specific germ detection using differential refraction index measurement > visual evaluation

  • University of Potsdam

  • von Nickisch-Rosenegk M, Marschan X, Andresen D, Bier FF. Reverse transcription-polymerase chain reaction on a microarray: the integrating concept of »active arrays«. Analytical and Bioanalytical Chemistry 08/2008; 391(5):1671-8.
  • Andresen D, von Nickisch-Rosenegk M, Bier FF. Helicase-dependent amplification: use in OnChip amplification and potential for point-of-care diagnostics. Expert Review of Molecular Diagnostics 10/2009; 9(7):645-50.
  • Andresen D, von Nickisch-Rosenegk M, Bier FF. Helicase dependent OnChip-amplification and its use in multiplex pathogen detection.Clinica chimica acta; international journal of clinical chemistry 04/2009; 403(1-2):244-8.
  • Danckert L, Hoppe S, Bier FF, von Nickisch-Rosenegk M. Rapid identification of novel antigens of Salmonella Enteritidis by microarray-based immunoscreening. Microchimica Acta 02/2014; 3.43 Impact Factor.
  • Herbel S, von Nickisch-Rosenegk M, Kuhn M, Murugaiyan J, Wieler LH, Guenther S. Specific TaqMan Probes for the Identification and Quantification of Lactobacilli in Pharmaceuticals. Journal of Probiotics & Health. 02/2014; 2(1).
  • Hoppe S, Bier FF, von Nickisch-Rosenegk M. Identification of antigenic proteins of the nosocomial pathogen Klebsiella pneumoniae. PLoS One 9(10): e110703. Doi dx.doi.org/10.1371/journal.pone.0110703.
  • Kersting S, Rausch V, Bier FF, von Nickisch-Rosenegk M. Rapid detection of Plasmodium falciparum with isothermal recombinase polymerase amplification and lateral flow analysis. Malaria Journal 03/2014; 13(1):99.
  • Kersting S, Rausch V, Bier FF, von Nickisch-Rosenegk M. Multiplex isothermal solid-phase recombinase polymerase amplification for the specific and fast DNA-based detection of three bacterial pathogens. Microchimica Acta 02/2014.