ivD-platform

The Fraunhofer ivD-platform consists of a cartridge the size of a credit card (»lab-on-a-chip«) and a base station. Blood, or other biological fluids, can be placed directly onto the cartridge, and are then able to be analyzed automatically in the base station within 10 to 15 minutes. The use of microarrays makes multiparameter diagnosis possible, and also allows a range of capture molecules to be used, such as antibodies, proteins, peptides, glycans or oligonucleotides. For its customers and partners, the institute is able to transfer existing tests (e.g. ELISAs, DNA microarrays, etc.) onto the ivD-platform, to optimize them and provide technical verification, and even ivD certification.

Methods

  • Assay development for serological assays
  • Assay development for blood, urine and saliva samples, as well as other media
  • Multiplex assays for up to 500 analytes
  • DNA-based assays (microarray)
  • PCR and RT-PCR development
  • Antibody assays
  • Dispensation of a range of different biological samples in different volumes/viscosities
  • Linkage of DNA, RNA, proteins and peptides to modified surfaces (glass, gold, silicon, polymer substrates, etc.)
  • Aptamers for in-vitro diagnostics
  • Biomolecular interaction analysis, kinetic analysis
  • Kinetic ranking of interactions using a Biacore Flexchip (peptide antibodies, protein DNA, etc.)
  • Characterization and validation of antibodies (cross-reactivity, specificity)
  • Serum screening
  • Aptamer assays
  • Assay development for biochips, microarray-based assays
  • Feasibility studies for alternative detection systems

 

Equipment

  • S1/S2 laboratories, assay development for pathogen detection
  • Multiplex assays
  • Microarray facilities, spotting: Biochip arrayer for the production of biochips (GenePix, non-contact, Scienion S5 & S7, TopSpotter, i2 from M2)
  • Scanner technology (Axon, Tecan)
  • Label-free biosensors (Biacore Flexchip, T100)
  • Fluorescence MTP reader
  • Nanoliter microdispenser
  • Hybridization station for semi-automatic microarray processing

  • Bader D, Klier DT, Hettrich C, Bier FF, Wessig P. Detecting carbohydrate–lectin interactions using a fluorescent probe based on DBD dyes. Anal. Methods. 2016 Jan 13; 8, 1235-1238: DOI dx.doi.org/10.1039/C5AY02991K
  • Hoppe S, Bier FF, von Nickisch-Rosenegk M. Identification of antigenic proteins of the nosocomial pathogen Klebsiella pneumoniae. PLoS One 9(10): e110703. Doi dx.doi.org/10.1371/journal.pone.0110703.
  • Scheller FW, Yarman A, Bachmann T, Hirsch T, Kubick S, Renneberg R, Schumacher S, Wollenberger U, Teller C, Bier FF. Future of Biosensors: A Personal View. Adv Biochem Eng Biotechnol. 140 (2014):1-28.
  • Kozma P, Kehl F, Ehrentreich-Förster E, Stamm C, Bier FF. Integrated planar optical waveguide interferometer biosensors: A comparative review. Biosens. Bioelectron. 58 (2014):287-307.
  • Kersting S, Rausch V, Bier FF, von Nickisch-Rosenegk M. Rapid detection of Plasmodium falciparum with isothermal recombinase polymerase amplification and lateral flow analysis. Malaria J., 13 (2014):99. (e1-e9).
  • Kersting S, Rausch V, Bier FF, von Nickisch-Rosenegk M. Multiplex isothermal solid-phase recombinase polymerase amplification for the specific and fast DNA-based detection of three bacterial pathogens. Mikrochim Acta. 181 (2014):1715-1723.
  • Bier FF, Schumacher S. Integration in Bioanalysis: Technologies for Point-of-Care Testing. Adv Biochem Eng Biotechnol. 133 (2013): 1-14.
  • Kozma P, Lehmann A, Wunderlich K, Michel D, Schu¬macher S, Ehrentreich-Förster E, Bier FF. A novel handheld fluorescent microarray reader for point-of-care diagnostic. Biosensors and Bioelectronics 47 (2013): 415–420.
  • Schumacher S, Lüdecke C, Ehrentreich-Förster E, Bier FF. Platform Technologies for Molecular Diagnostics Near the Patient's Bedside. Adv Biochem Eng Biotechnol. 133 (2013): 75-87.
  • Schumacher S, Sartorius D, Ehrentreich?Förster E, Bier FF. Miniaturization for Point-of-Care analysis: Platform technology for almost every biomedical assay. The Journal of the International Federation of Clinical Chemistry and Laboratory Medicine 23 (2012), 1-6.
  • Hoppe S, Bier FF, von Nickisch-Rosenegk M. Microarray-based method for screening of immunogenic proteins from bacteria. J Nanobiotechnology. 10 (2012): e12 (1-8).
  • von Nickisch-Rosenegk M, Teschke T, Bier FF. Construction of an artificial cell membrane anchor using DARC as a fitting for artificial extracellular functionalities of eukaryotic cells. J Nanobiotechnology 10 (2012): e1 (1-7).
  • Schumacher S, Nestler J, Otto T, Wegener M, Ehrentreich-Förster E, Michel D, Wunderlich K, Palzer S, Sohn K, Weber A, Burgard M, Grzesiak A, Teichert A, Brandenburg A, Koger B, Albers J, Nebling E, Bier FF. Highly-integrated lab-on-chip system for point-of-care multiparameter analysis. Lab Chip. 12 (2012): 464-473.
  • Peter H, Berggrav K, Thomas P, Pfeifer Y, Witte W, Templeton K, Bachmann TT. Direct detection and genotyping of Klebsiella pneumoniae carbapenemases from urine by use of a new DNA microarray test. J. Clin. Microbiol (2012) 50 (12), S. 3990–3998.
  • Reiss E, Hölzel R, Bier FF. Preparation of DNA nanostructures with repetitive binding motifs by rolling circle amplification. Methods Mol. Biol (2011) 749, S. 151–168.