The research field deals primarily with the development of specific assays for the validation of biomarkers as well as the development and adaptation of assays for a broad range of platforms, such as microarrays, ELISA, lateral-flow systems as well as beads-based assays in the life sciences and environmental and food analytics. In addition, physico-chemical parameters such as kinetic constants (KD) with marker-free detection methods (e.g. Biacore, bScreen, Nanotemper) and the qualities or modification of surfaces (e.g. contact angle measurements and ellipsometry) can be identified. All technologies are continuously further developed for specific (custom) applications. These include system-biological projects for validating potential biomarkers, the kinetic analysis of antibodies, the quantification of specific markers in serum samples, as well as the development of point-of-need applications, e.g. for identifying microbial loads in environmental samples.
- Production (spotting) of custom DNA, peptide and protein microarrays
- Spotting on various materials, e.g. glass, plastic, membranes, microtiter plates, conductors, etc.
- Benchmarking of various contact and non-contact spotters for selecting the ideal system (reference laboratory for liquid dispensing systems)
- Carrying out customer-specific microarray experiments, evaluation and documentation
- Optimizing the experiments with thermodynamic and kinetic measurements
- Transferring other assay formats to microarrays and lateral flow test
- Developing and establishing assays for ELISA and microarrays
- Colorimetric, fluorescence and electrochemical detection systems
- Serum screening for identifying disease-associated antibody profiles
- Detection and validation of (potential) biomarkers in bodily fluids
- Epitomapping of antibodies
- Bead-based assays (Luminex platform)
- Design of experiment (DoE)