Cell microarrays

The application of defined quantities of cells onto specific sites is an important topic within cellular biotechnology, for example in the field of tissue engineering, cell analysis and transfection arrays. The controlled dispensation of living cells onto the corresponding substrate is decisive for these processes. With the technology available at the institute, living cells can be dispensed onto specific surfaces in order to use them for further research. The cells have a survival rate of up to 95%.  This means that a technology is available that allows a large range of different areas of inquiry to be addressed. Transfection arrays involve dispensing the cells onto a mixture of transfection reagent and DNA, which causes the cells to absorb the DNA. The DNA may be, for example, protein coding vectors or RNAi constructs. Following transfection, the cells can be characterized. This method makes it possible to test a large number of DNA constructs in parallel and under identical conditions. Another application for cell microarrays is the dispensation of different cell types onto (pre)structured surfaces. On the one hand, this allows the interaction of the cells to be examined and characterized, while on the other hand, it allows tissue-like structures and cell populations to be created.