DNA and RNA microarrays/expression analysis

To understand the complex regulatory mechanisms and to study cellular mechanisms, it is essential to carry out a simultaneous analysis of the expression of all transcriptions of a cell, a cell population, tissue or organism for a specific period of time. The complete genomic sequence of many organisms, such as humans, mice, Drosophila melanogaster and Caenorhabditis elegans are now available. Using second generation sequencing, information about transcription activity within an organism can be collected within a few days. The method makes it possible to identify genes that are required for complex development networks and signal transduction pathways.

Based on this information, specific DNA/RNA microarrays can be produced to identify changes in certain significant transcriptions. Based on this information, patients can, for example, be classified/stratified or tests can be developed to help choose treatments and support their effectiveness.

The focus is on examining human cancer material. Information is collected to enable early diagnosis, a precise prognosis and the identification of potentially useful treatments, as well to assess the effectiveness of the treatment. In addition to analyzing RNA transcriptions, it is also possible to identify microRNAs as well as epigenetic changes.