Fraunhofer-Institut für Zelltherapie und Immunologie, Institutsteil Bioanalytik und Bioprozesse IZI-BB- Bazzone, Andre; Körner, Alexander; Meincke, Melanie; Bhatt, Manan; Dondapati, Srujan; Barthmes, Maria; Kubick, Stefan; Fertig, Niels. SSM-based electrophysiology, a label-free real-time method reveals sugar binding & transport events in SGLT1. Biosensors and Bioelectronics. 2022. doi.org/10.1016/j.bios.2021.113 PDF
- Dreymann, Nico; Sabrowski, Wiebke; Danso, Jennifer; Menger, Marcus M. Aptamer-Based Sandwich Assay Formats for Detection and Discrimination of Human High- and Low-Molecular-Weight uPA for Cancer Prognosis and Diagnosis. Cancers. 2022. doi.org/10.3390/cancers14215222 PDF
- Flechner, Marie; Schaller, Julia; Stahl, Maike; Achberger, Kevin; Gerike, Susanna; Hannappel, Yvonne; Fu, Jianan; Jaeger, Magnus; Hellweg, Thomas; Duschl, Claus; Uhlig, Katja. Adhesion, proliferation, and detachment of various cell types on thermoresponsive microgel coatings. Biotechnology and Bioengineering. 2022. doi: 10.1002/bit.28095
- Haueis, Lisa; Stech, Marlitt; Kubick, Stefan. A Cell-free Expression Pipeline for the Generation and Functional Characterization of Nanobodies. Frontiers in Bioengineering and Biotechnology. 2022. 10.3389/fbioe.2022.896763. doi: 10.3389/fbioe.2022.896763 PDF
- Knauer, Jan Felix; Liers, Christiane; Hahn, Stephanie; Wuestenhagen, Doreen A.; Zemella, Anne; Kellner, Harald; Haueis, Lisa; Hofrichter, Martin; Kubick, Stefan. Cell-free production of the bifunctional glycoside hydrolase GH78 from Xylaria polymorpha. Enzyme and Microbial Technology. 2022. doi:10.1016/j.enzmictec.2022.110110 PDF
- Krebs, Simon K.; Rakotoarinoro, Nathanaël; Stech, Marlitt; Zemella, Anne; Kubick, Stefan. A CHO-Based Cell-Free Dual Fluorescence Reporter System for the Straightforward Assessment of Amber Suppression and scFv Functionality. Frontiers in Bioengineering and Biotechnology. 2022. doi: 10.3389/fbioe.2022.873906 PDF
- Krebs, Simon K.; Stech, Marlitt; Jorde, Felix; Rakotoarinoro, Nathanaël; Ramm, Franziska; Marinoff, Sophie; Bahrke, Sven; Danielczyk, Antje; Wüstenhagen, Doreen A.; Kubick, Stefan. Synthesis of an Anti-CD7 Recombinant Immunotoxin Based on PE24 in CHO and E. coli Cell-Free Systems. International Journal of Molecular Sciences. 2022. DOI: 10.3390/ijms232213697.
- Ramm, Franziska; Dondapati, Srujan K.; Trinh, Hoai Anh; Wenzel, Dana; Walter, Ruben M.; Zemella, Anne; Kubick, Stefan. The Potential of Eukaryotic Cell-Free Systems as a Rapid Response to Novel Zoonotic Pathogens: Analysis of SARS-CoV-2 Viral Proteins. Frontiers in Bioengineering and Biotechnology. 2022. 10.3389/fbioe.2022.896751. doi: 10.3389/fbioe.2022.896751 PDF
- Ramm, Franziska; Jack, Lena; Kaser, Danny; Schloßhauer, Jeffrey L.; Zemella, Anne; Kubick, Stefan. Cell-Free Systems Enable the Production of AB5 Toxins for Diagnostic Applications. Toxins. 2022. 10.3390/toxins14040233. doi: 10.3390/toxins14040233 PDF
- Schloßhauer, Jeffrey L.; Cavak, Niño; Zemella, Anne; Thoring, Lena; Kubick, Stefan. Cell Engineering and Cultivation of Chinese Hamster Ovary Cells for the Development of Orthogonal Eukaryotic Cell-free Translation Systems. Frontiers in Molecular Biosciences. 2022. 10.3389/fmolb.2022.832379. doi: 10.3389/fmolb.2022.832 PDF
- Walter, Ruben Magnus; Zemella, Anne; Schramm, Marina; Kiebist, Jan; Kubick, Stefan. Vesicle-based cell-free synthesis of short and long unspecific peroxygenases. Frontiers in Bioengineering and Biotechnology. 2022. 10.3389/fbioe.2022.964396. doi: 10.3389/fbioe.2022.964396