Fraunhofer-Institut für Zelltherapie und Immunologie, Institutsteil Bioanalytik und Bioprozesse IZI-BB
Fraunhofer-Institut für Zelltherapie und Immunologie, Institutsteil Bioanalytik und Bioprozesse IZI-BB

Publikationen 2022

  1. Agarwal, S.; Warmt, C.; Henkel, J.; Schrick, L.; Nitsche, A.; Bier, FF. Lateral flow–based nucleic acid detection of SARS‑CoV‑2 using enzymatic incorporation of biotin‑labeled dUTP for POCT use. Analytical and Bioanalytical Chemistry 414 (2022), 3177–3186. doi: 10.1007/s00216-022-03880-4.
  2. Blazquez-Navarro A., Bauer C., Wittenbrink N., Wolk K., Sabat R., Dang-Heine C., Neumann S., Roch T., Wehler P., Blazquez-Navarro R., Olek S., Thomusch O., Seitz H., Reinke P., Hugo C., Sawitzki B., Babel N. and Or-Guil M. Early prediction of renal graft function: Analysis of a multi-centre, multi-level data set. Curr Res Transl Med. 2022 Jul;70(3):103334. 10.1016/j.retram.2022.103334
  3. Bazzone, Andre; Körner, Alexander; Meincke, Melanie; Bhatt, Manan; Dondapati, Srujan; Barthmes, Maria; Kubick, Stefan; Fertig, Niels. SSM-based electrophysiology, a label-free real-time method reveals sugar binding & transport events in SGLT1. Biosensors and Bioelectronics. 2022.  doi.org/10.1016/j.bios.2021.113 PDF
  4. Dreymann, Nico; Sabrowski, Wiebke; Danso, Jennifer; Menger, Marcus M. Aptamer-Based Sandwich Assay Formats for Detection and Discrimination of Human High- and Low-Molecular-Weight uPA for Cancer Prognosis and Diagnosis. Cancers. 2022. doi.org/10.3390/cancers14215222 PDF
  5. Dreymann N.; Wuensche J.; Sabrowski W.; Moeller A.; Czepluch D.; Vu Van D.; Fuessel S.; Menger M.M. Inhibition of Human Urokinase-Type Plasminogen Activator (uPA) Enzyme Activity and Receptor Binding by DNA Aptamers as Potential Therapeutics through Binding to the Different Forms of uPA. Mol Sci. 2022 Apr 28;23(9):4890. doi:10.3390/ijms23094890
  6. Dreymann, N.; Möller, A.; Menger, M.M. Label-Free Determination of the Kinetic Parameters of Protein-Aptamer Interaction by Surface Plasmon Resonance. In: Mayer, G., Menger, M.M. (eds) Nucleic Acid Aptamers. Methods in Molecular Biology, vol 2570. Humana, New York, NY. doi.org/10.1007/978-1-0716-2695-5_11
  7. Flechner, Marie; Schaller, Julia; Stahl, Maike; Achberger, Kevin; Gerike, Susanna; Hannappel, Yvonne; Fu, Jianan; Jaeger, Magnus; Hellweg, Thomas; Duschl, Claus; Uhlig, Katja. Adhesion, proliferation, and detachment of various cell types on thermoresponsive microgel coatings. Biotechnology and Bioengineering. 2022. doi: 10.1002/bit.28095
  8. Haueis, Lisa; Stech, Marlitt; Kubick, Stefan. A Cell-free Expression Pipeline for the Generation and Functional Characterization of Nanobodies. Frontiers in Bioengineering and Biotechnology. 2022. 10.3389/fbioe.2022.896763. doi: 10.3389/fbioe.2022.896763 PDF
  9. Hofrichter M.; Kellner H.; Herzog R.; Karich A.; Kiebist J.; Scheibner K.; Ullrich R. Peroxide-Mediated Oxygenation of Organic Compounds by Fungal Peroxygenases. Antioxidants, 2022, Vol. 11 (1), S. 163. doi: 10.3390/antiox11010163
  10. Kerler, Y.; Sass, S.; Hille, C.; Menger, M.M. Determination of Aptamer Structure Using Circular Dichroism Spectroscopy.  In: Mayer, G., Menger, M.M. (eds) Nucleic Acid Aptamers. Methods in Molecular Biology, vol 2570. Humana, New York, NY. doi.org/10.1007/978-1-0716-2695-5_9
  11. Knauer, Jan Felix; Liers, Christiane; Hahn, Stephanie; Wuestenhagen, Doreen A.; Zemella, Anne; Kellner, Harald; Haueis, Lisa; Hofrichter, Martin; Kubick, Stefan. Cell-free production of the bifunctional glycoside hydrolase GH78 from Xylaria polymorpha. Enzyme and Microbial Technology. 2022. doi:10.1016/j.enzmictec.2022.110110 PDF
  12. Koenig R.; Kiebist J.; Kalmbach J.; Herzog R.; Schmidtke K-U.; Kellner H.; Ullrich R, Jehmlich N.; Hofrichter M.; Scheibner K. Novel unspecific peroxygenase from Truncatella angustata catalyzes the synthesis of bioactive lipid mediators. Microorganisms, 2022, Vol. 10 S. 126. doi: 10.3390/microorganisms10071267
  13. Krebs, Simon K.; Rakotoarinoro, Nathanaël; Stech, Marlitt; Zemella, Anne; Kubick, Stefan. A CHO-Based Cell-Free Dual Fluorescence Reporter System for the Straightforward Assessment of Amber Suppression and scFv Functionality. Frontiers in Bioengineering and Biotehnology. 2022. doi: 10.3389/fbioe.2022.873906 PDF
  14. Krebs, Simon K.; Stech, Marlitt; Jorde, Felix; Rakotoarinoro, Nathanaël; Ramm, Franziska; Marinoff, Sophie; Bahrke, Sven; Danielczyk, Antje; Wüstenhagen, Doreen A.; Kubick, Stefan. Synthesis of an Anti-CD7 Recombinant Immunotoxin Based on PE24 in CHO and E. coli Cell-Free Systems. International Journal of Molecular Sciences. 2022.  DOI: 10.3390/ijms232213697.
  15. Kruse, M.; Altattan, B.; Laux, E-M.; Grasse, N.; Heinig, L.; Möser, C.; Smith, DM.; Hölzel, R. Characterization of binding interactions of SARS-CoV-2 spike protein and DNA-peptide nanostructures. Scientific Reports 12 (2022), 12828. https://doi.org/10.1038/s41598-022-16914-9
  16. Kruse, M.; Möser, C.; Smith, DM.; Müller-Landau, H.; Rant, U.; Hölzel, R.; Bier, FF. Measuring Influenza A Virus and Peptide Interaction Using Electrically Controllable DNA Nanolevers. Advanced Materials Technologies 7 (2022), 2101141. doi.org/10.1002/admt.202101141
  17. Leya T.  The CCCryo Culture Collection of Cryophilic Algae as a valuable bioresource for algal biodiversity and for novel, industrially marketable metabolites. Applied Phycology 3(1) (2022): 167-188. doi.org/10.1080/26388081.2020.1753572
  18. Liu Y.; Jeraldo P.; Herbert W.; McDonough S.; Eckloff B.; de Vera J-P.; Cockell C.; Leya T.; Baqué M.; Jen J.; Schulze-Makuch D.; Walther-Antonio M. Non-random genetic alterations in the cyanobacterium Nostoc sp. exposed to space conditions. Sci. Rep. 12 (2022): 12580. doi.org/10.1038/s41598-022-16789-w
  19. Liu Y.; Jeraldo P.; Herbert W.; McDonough S.; Eckloff B.; Schulze-Makuch D.; de Vera J-P.; Cockell C.; Leya T.; Baqué M.; Jen J.; Walther-Antonio M. Whole genome sequencing of cyanobacterium Nostoc sp. CCCryo 231-06 using microfluidic single cell technology. iScience 25(5) (2022): 104291. doi.org/10.1016/j.isci.2022.104291
  20. Major, S.; Gajovic-Eichelmann, N.; Woitzik, J.; Dreier, J P. Oxygen-Induced and pH-Induced Direct Current Artifacts on Invasive Platinum/Iridium Electrodes for Electrocorticography. Neurocritical Care 35(2021), 146. DOI: 10.1007/s12028-021-01358-2
  21. Mattig E.; Guest PC.; Peter H. A Rapid User-Friendly Lab-on-a-Chip Microarray Platform for Detection of SARS-CoV-2 Variants. Methods Mol Biol. 2022;2511:117-131. doi.org/10.1007/978-1-0716-2395-4_9
  22. Mayer, G.; Menger, M.M. (eds). "Nucleic Acid Aptamers. Selection, Characterization, and Application". Methods in Molecular Biology (MIMB, volume 2570). Humana, New York, NY. doi.org/10.1007/978-1-0716-2695-5
  23. Pellegrino, Antonio; Mükusch,Sandra; Seitz, Viola; Stein, Christopher; Herberg,Friedrich W.; Seitz, Harald. Transient receptor potential vanilloid 1 signaling is independent on Protein kinase A phosphorylation of Ankyrin-rich membrane spanning protein. Med. Sci. 2022, 10, 63. doi.org/10.3390/medsci10040063
  24. Peter A.; Mattig E.; Guest PC.; Bier FF. Lab-on-a-Chip Immunoassay for Prediction of Severe COVID-19 Disease. Methods Mol Biol. 2022;2511:235-244. doi.org/10.1007/978-1-0716-2395-4_17
  25. Prüfer, M.; Wenger, C.; Bier, FF.; Laux, E-M.; Hölzel, R. Activity of AC electrokinetically immobilized horseradish peroxidase. Electrophoresis, 43 (2022), 1920. DOI:10.1002/elps.202200073
  26. Ramm, Franziska; Dondapati, Srujan K.; Trinh, Hoai Anh; Wenzel, Dana; Walter, Ruben M.; Zemella, Anne; Kubick, Stefan. The Potential of Eukaryotic Cell-Free Systems as a Rapid Response to Novel Zoonotic Pathogens: Analysis of SARS-CoV-2 Viral Proteins. Frontiers in Bioengineering and Biotechnology. 2022. 10.3389/fbioe.2022.896751. doi: 10.3389/fbioe.2022.896751 PDF 
  27. Ramm, Franziska; Jack, Lena; Kaser, Danny; Schloßhauer, Jeffrey L.; Zemella, Anne; Kubick, Stefan. Cell-Free Systems Enable the Production of AB5 Toxins for Diagnostic Applications. Toxins. 2022. 10.3390/toxins14040233.  doi: 10.3390/toxins14040233 PDF
  28. Sabrowski W.; Dreymann N.; Möller A.; Czepluch D.; Albani P.P.; Theodoridis D.; Menger M.M. The use of high-affinity polyhistidine binders as masking probes for the selection of an NDM-1 specific aptamer. Sci Rep. 2022 May 13;12(1):7936. doi: 10.1038/s41598-022-12062-2
  29. Sabrowski, W.; Stöcklein, W.F.M.: Menger, M.M.  Immobilization-Free Determination of Dissociation Constants Independent of Ligand Size Using MicroScale Thermophoresis. Mayer, G., Menger, M.M. (eds) Nucleic Acid Aptamers. Methods in Molecular Biology, vol 2570. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2695-5_10
  30. Schloßhauer, Jeffrey L.; Cavak, Niño; Zemella, Anne; Thoring, Lena; Kubick, Stefan. Cell Engineering and Cultivation of Chinese Hamster Ovary Cells for the Development of Orthogonal Eukaryotic Cell-free Translation Systems. Frontiers in Molecular Biosciences. 2022. 10.3389/fmolb.2022.832379. doi: 10.3389/fmolb.2022.832 PDF 
  31. Schmidt C.; Kammel A.; Tanner J.A.; Kinghorn A.B.; Khan M.M.; Lehmann W.; Menger M.; Schedler U.; Schierack P.; Rödiger S. A multiparametric fluorescence assay for screening aptamer–protein interactions based on microbeads. Sci Rep. 2022 Feb 22;12(1):2961. 10.1038/s41598-022-06817-0
  32. Schramm M.; Friedrich S.; Schmidtke K-U.; Kiebist J.; Panzer P.; Kellner H.; Ullrich R.; Hofrichter M.; Scheibner K. Cell-Free Protein Synthesis with Fungal Lysates for the Rapid Production of Unspecific Peroxygenases. Antioxidants, 2022, Vol. 11 (2), S. 284. doi: 10.3390/antiox11020284
  33. Stanke, S.; Wenger, C.; Bier, FF.; Hölzel, R. AC electrokinetic immobilization of influenza virus. Electrophoresis 43 (2022), 1309. doi: 10.1002/elps.202100324
  34. Ullrich J.; Göhmann P.J.; Zemella A.; Kubick S. Oligomerization of the heteromeric γ-aminobutyric acid receptor GABAB in a eukaryotic cell-free system. Sci Rep. 2022 Dec 1;12(1):20742. doi: 10.1038/s41598-022-24885-0
  35. Walter, Ruben Magnus; Zemella, Anne; Schramm, Marina; Kiebist, Jan; Kubick, Stefan. Vesicle-based cell-free synthesis of short and long unspecific peroxygenases. Frontiers in Bioengineering and Biotechnology. 2022. 10.3389/fbioe.2022.964396. doi: 10.3389/fbioe.2022.964396
  36. Warmt, C.; Yaslanmaz, C.; Henkel, J. Investigation and validation of labelling loop mediated isothermal amplification (LAMP) products with different nucleotide modifications for various downstream analysis. Scientific Reports 12 (2022), 7137. doi: 10.1038/s41598-022-11320-7