Fraunhofer-Institut für Zelltherapie und Immunologie, Institutsteil Bioanalytik und Bioprozesse IZI-BB
Fraunhofer-Institut für Zelltherapie und Immunologie, Institutsteil Bioanalytik und Bioprozesse IZI-BB

Dr. Anne Zemella

 

© Fraunhofer IZI-BB

Wissenschaftlicher Fokus und Expertise:

Alle ausklappen Alle einklappen

Anne Zemella - ORCID: 0000-0003-4895-5423
  • Cooper et al. "EXPERT" A Practical and Forward-Looking Guide to Capturing Protein Expression and Purification Metadata to develop Machine Learning Models. J Biol Chem. 2026 Sep 29:113610. 10.1016/j.jbc.2026.113610
  • Grimm AK, Balk S, Göpferich A, Breunig M, Aubele S, Zemella A, Wegener J. Localized delivery of coding nucleic acids into adherent cells by in situ electroporation: integrated impedance-based monitoring allows for loss-of-function or gain-of-function screening. Sci Rep. 2026 Jul 29;16(1):23544. 10.1038/s41598-026-63882-5
  • Hartung T, Freyer D, Zemella A, Radbruch H, Weiner J, El-Din JJ, Meisel A, Priller J. Non-hematopoietic erythropoietin splice variant is produced in the diseased human brain and confers neuroprotection. Front Cell Neurosci. 2026;19:1677505. doi: 10.3389/fncel.2025.1677505
  • Hartung T, Zemella A, Meisel A. Protocol to validate custom-designed BaseScope probes using cell-free synthesized protein lysates and in vitro-transcribed purified mRNA. STAR Protoc. 2025;6(4):104180. DOI: 10.1016/j.xpro.2025.104180
  • Casteleijn MG, Abendroth U, Zemella A, Walter R, Rashmi R, Haag R, Kubick S. Beyond in vivo, pharmaceutical molecule production in cell-free systems and the use of noncanonical amino acids therein. Chem Rev. 2025;125(3):1303–1331. DOI: 10.1021/acs.chemrev.4c00126
  • Behrens L, Walter RM, Cai W, Ewers H, van Bommel B, Zemella A. Fast in vitro synthesis and direct labeling of nanobodies for prototyping in microscopy applications. ACS Omega. 2024;9(33):35374–35383. DOI: 10.1021/acsomega.4c01164
  • Schloßhauer JL, Tholen L, Körner A, Kubick S, Chatzopoulou S, Hönow A, Zemella A. Promoting the production of challenging proteins via induced expression in CHO cells and modified cell-free lysates harboring T7 RNA polymerase and mutant eIF2α. Synth Syst Biotechnol. 2024;9(3):416–424. DOI: 10.1016/j.synbio.2024.03.011
  • Ramm F, Kaser D, König I, Fellendorf J, Wenzel D, Zemella A, Papatheodorou P, Barth H, Schmidt H. Synthesis of biologically active Shiga toxins in cell-free systems. Sci Rep. 2024;14(1):6043. DOI: 10.1038/s41598-024-56190-3
  • Zemella A, Beer K, Ramm F, Wenzel D, Düx A, Merkel K, Calvignac-Spencer S, Stern D, Dorner MB, Dorner BG, Widulin N, Schnalke T, Walter C, Wolbert A, Schmid BG, Mankertz A, Santibanez S. Vaccine-induced neutralizing antibodies bind to the H protein of a historical measles virus. Int J Med Microbiol. 2024;314:151607. DOI: 10.1016/j.ijmm.2024.151607
  • Ullrich J, Haueis L, Ohlhoff C, Zemella A, Kubick S, Stech M. Solubilization of oligomeric cell-free synthesized proteins using SMA copolymers. Methods Mol Biol. 2024;2762:293–308. DOI: 10.1007/978-1-0716-3666-4_18
  • Schloßhauer JL, Dondapati SK, Kubick S, Zemella A. A cost-effective Pichia pastoris cell-free system driven by glycolytic intermediates enables the production of complex eukaryotic proteins. Bioengineering (Basel). 2024;11(1):92. DOI: 10.3390/bioengineering11010092
  • Knauer JF, Schulz C, Zemella A, Wüstenhagen DA, Walter RM, Küpper JH, Kubick S. Synthesis of mono cytochrome P450 in a modified CHO-CPR cell-free protein production platform. Sci Rep. 2024;14(1):1271. DOI: 10.1038/s41598-024-51781-6
  • Ullrich, J., Ohlhoff, C., Dondapati, S. K., Zemella, A., & Kubick, S. (2023). Evaluation of the Ion Channel Assembly in a Eukaryotic Cell-Free System Focusing on Two-Pore Domain Potassium Channels K2P. International Journal of Molecular Sciences, 24(7). https://doi.org/10.3390/ijms24076299
  • Schloßhauer, J. L., Zemella, A., Dondapati, S. K., Thoring, L., Meyer, M., & Kubick, S. (2023). Enhancing the performance of a mutant pyrrolysyl-tRNA synthetase to create a highly versatile eukaryotic cell-free protein synthesis tool. Scientific Reports, 13(1), 15236. https://doi.org/10.1038/s41598-023-42198-8
  • Pouyan, P., Zemella, A., Schloßhauer, J. L., Walter, R. M., Haag, R., & Kubick, S. (2023). One to one comparison of cell-free synthesized erythropoietin conjugates modified with linear polyglycerol and polyethylene glycol. Scientific Reports, 13(1), 6394. https://doi.org/10.1038/s41598-023-33463-x
  • Haueis, L., Stech, M., Schneider, E., Lanz, T., Hebel, N., Zemella, A., & Kubick, S. (2023). Rapid One-Step Capturing of Native, Cell-Free Synthesized and Membrane-Embedded GLP-1R. International Journal of Molecular Sciences, 24(3). https://doi.org/10.3390/ijms24032808
  • Ullrich, J.; Göhmann, P. J.; Zemella, A.; Kubick, S. (2022) Oligomerization of the heteromeric γ-aminobutyric acid receptor GABAB in a eukaryotic cell-free system. Scientific reports. 10.1038/s41598-022-24885-0.
  • Schloßhauer, J. L.; Cavak, N.; Zemella, A.; Thoring, L.; Kubick, S. (2022) Cell Engineering and Cultivation of Chinese Hamster Ovary Cells for the Development of Orthogonal Eukaryotic Cell-free Translation Systems. Frontiers in Molecular Biosciences. 10.3389/fmolb.2022.832379.
  • Ramm, F.; Jack, L.; Kaser, D.; Schloßhauer, J. L.; Zemella, A.; Kubick, S. (2022) Cell-Free Systems Enable the Production of AB5 Toxins for Diagnostic Applications. Toxins. 10.3390/toxins14040233.
  • Ramm, F.; Dondapati, S. K.; Trinh, H. A.; Wenzel, D.; Walter, R. M.; Zemella, A.; Kubick, S. (2022) The Potential of Eukaryotic Cell-Free Systems as a Rapid Response to Novel Zoonotic Pathogens: Analysis of SARS-CoV-2 Viral Proteins. Frontiers in Bioengineering and Biotechnology. 10.3389/fbioe.2022.896751.
  • Knauer, J. F.; Liers, C.; Hahn, S.; Wuestenhagen, D. A.; Zemella, A.; Kellner, H.; Haueis, L.; Hofrichter, M.; Kubick, S. (2022) Cell-free production of the bifunctional glycoside hydrolase GH78 from Xylaria polymorpha. Enzyme and Microbial Technology. 10.1016/j.enzmictec.2022.110110.
  • Walter, R. M.; Zemella, A.; Schramm, M.; Kiebist, J.; Kubick, S. (2022) Vesicle-based cell-free synthesis of short and long unspecific peroxygenases. Frontiers in Bioengineering and Biotechnology. 10.3389/fbioe.2022.964396.
  • Krebs, S. K.; Rakotoarinoro, N.; Stech, M.; Zemella, A.; Kubick, S. (2022) A CHO-Based Cell-Free Dual Fluorescence Reporter System for the Straightforward Assessment of Amber Suppression and scFv Functionality. Frontiers in Bioengineering and Biotechnology. https://doi.org/10.3389/fbioe.2022.873906.
  • Ramm, F.; Stech, M.; Zemella, A.; Frentzel, H.; Kubick, S. (2021) The Pore-Forming Hemolysin BL Enterotoxin from Bacillus cereus: Subunit Interactions in Cell-Free Systems. Toxins. 10.3390/toxins13110807.
  • Stech, M.; Rakotoarinoro, N.; Teichmann, T.; Zemella, A.; Thoring, L.; Kubick, S. (2021) Synthesis of Fluorescently Labeled Antibodies Using Non-Canonical Amino Acids in Eukaryotic Cell-Free Systems. Methods in molecular biology (Clifton, N.J.). 10.1007/978-1-0716-1406-8_9.
  • Dhandapani, P.; Dondapati, S. K.; Zemella, A.; Bräuer, D.; Wüstenhagen, D. A.; Mergler, S.; Kubick, S. (2021) Targeted esterase-induced dye (TED) loading supports direct calcium imaging in eukaryotic cell-free systems. RSC advances. 10.1039/d0ra08397f.
  • Dondapati, S.K., STECH, M., M., ZEMELLA, A., KUBICK, S. (2020) Cell‑Free Protein Synthesis: A Promising Option for Future Drug Development. BioDrugs , https://doi.org/10.1007/s40259-020-00417-y
  • Zaitseva, E., Dondapati, S., Schloßhauer, J., Zemella, A., Dhandapani, P., Kubick, S., Baaken, G. (2020) Functional Characerization of Ion Channels Expressed in Eukaryotic Cell-free Systems using Lipid Bilayer Arrays. Biophysical Journal, Volume 118, Issue 3, Supplement 1, 586A.
  • Ramm, F., Dondapati, S.K., Thoring L., Zemella A., Wüstenhagen, D.A., Frentzel, H., Stech M., Kubick, S. (2020) Mammalian cell-free protein expression promotes the functional characterization of the tripartite non-hemolytic enterotoxin from Bacillus cereus. Sci Rep. 10:2887.
  • Zemella A, Grossmann S, Sachse R, Sonnabend A, Schaefer M, Kubick S. (2017) Qualifying a eukaryotic cell-free system for fluorescence based GPCR analyses. Sci Rep. 7(1):3740. doi:10.1038/s41598-017-03955-8. Artikel
  • Zemella A, Thoring L, Hoffmeister C, Kubick S. (2015) Cell-Free Protein Synthesis: Pros and Cons of Prokaryotic and Eukaryotic Systems. Chembiochem. dx.doi.org/10.1002/cbic.201500340.