The field of activity includes the development of specific assays for the validation of biomarkers as well as the development and adaptation of assays to different platforms such as microarrays, ELISA, lateral flow systems and beads-based assays in the field of life science, environmental and food analysis. This includes the determination of physicochemical parameters such as kinetic constants (KD) with label-free detection methods (e.g. Biacore, bScreen, Nanotemper), the characterization of interactions e.g. antibody - antigen interactions and the acquisition of parameters that are important for assay development such as the nature or modification of surfaces (e.g. contact angle measurements and ellipsometry). The existing methods and techniques are continuously further developed for (customer-) specific applications. Based on the existing knowledge, specific training and education can be offered to external partners. Methods and techniques used include proteomics techniques such as beads-based methods (Luminex) and microarray but also genomics techniques such as 2nd generation sequencing and various nucleic acid amplification and quantification methods.
Applications include system biology projects for validation of potential biomarkers, kinetic analysis and characterization of antibodies, quantification of specific markers in serum samples such as cytokines and miRNAs, and development of point-of-need applications e.g. determination of microbial loads in environmental samples.