The evaluation of protein synthesis is an interesting R&D service for industrial research projects. Cell-free protein synthesis, often designated as in vitro translation, is a fast and efficient technique that achieves its goal with significantly less reaction steps than in vivo methods. With this method, proteins can be synthesized directly in their natural environment using lysates from cultured cell lines. Various cell-free systems based on lysates from pro- and eukaryotic resources allow the production of a wide range of differently structured and modified proteins. In the course of the project, different cell-free systems will be investigated to find the appropriate system for each specific problem. Special requirements such as glycosylation, membrane protein synthesis in native environment, disulfide bridging or signal sequence splitting are addressed.