Fraunhofer Institute for Cell Therapy and Immunology, Branch Bioanalytics and Bioprocesses IZI-BB
Fraunhofer Institute for Cell Therapy and Immunology, Branch Bioanalytics and Bioprocesses IZI-BB

Next-generation sequencing

Next Generation sequencing is based on the Sequence by Synthesis (SBS) principle. According to this principle, short molecules are synthesized and sequenced on a solid carrier. Fraunhofer IZI-BB is equipped with an Illumina MiSeq, which can run up to 25 million reads per run. This technology is very flexible in its fields of application. The sample spectrum includes the following substances.

  • DNA
  • cDNA
  • mRNA
  • Total RNA
  • micro RNA
  • 16 rRNA
  • Different nucleic acid species

Fields of application

  • Total genome sequencing of bacteria/viruses 
  • Metagenome analyses via 16S rRNA, e.g. for typing or diagnostics 
  • Transcriptome analyses, e.g. for drug screening, therapeutic applications, environmental influences, etc.
  • Exomanalyses 
  • Analyses of the microbiome for agriculture, environment, medicine 
  • Targeted sequencing of gene panels, e.g. for cancer research, biomarkers, autoimmune diseases, genetic diseases, forensics, etc.  
  • Environmental analysis e.g. from exotic sources  
  • Control of purity, e.g. in the food industry (meat or similar) or in other areas (tropical woods) 
  • Control of characteristics or parentage, e.g. for breeding horses, falcons, dogs, etc.

Expression Profiling

Targeted Gene Expression

Obtain a detailed view of your gene expression of interest. Targeted RNA-sequencing enables quantitative and qualitative information of hundreds of specific transcripts from multiple samples in a single sequencing run. Targeted Gene Expression can be useful for neuroscience, immunology and cancer research.

  • Running Projects: Expression of clock genes

 

miRNA & small RNA Analysis

Receive a detailed view of differentially expressed small noncoding RNAs. Small RNA sequencing is a technique to isolate and sequence small RNA species, such as microRNAs, which act in gene silencing and post-transcriptional regulation of gene expression. Small RNA Analysis can be useful in cancer research, biomarker identification and to examine post-transcriptional regulation from multiple samples in one assay

  • Running Projects: miRNA-profiling of polyneuropathy patients

 

Small Genome Sequencing

Whole-Genome Sequencing

Get a detailed genomic view of small organisms. Small genome sequencing (≤ 5 Mb) allows investigation of all genes of single bacteria, viruses or other microbes. Sequencing small microbial genomes can be useful for monitoring infectious diseases, molecular epidemiology studies, public health-related food testing and environmental metagenomics.

Targeted Gene Sequencing

Gain a detailed view of your genes of interest. Amplicon-based sequencing is a highly targeted approach that enables to analyze genetic variation of only a subset of genes or genome regions of research interest. Targeted gene sequencing can be useful for genetic disease, cancer and immunology research as well as plant and animal sequencing to analyze hundreds of target genomic regions from multiple samples in one assay.