Fraunhofer Institute for Cell Therapy and Immunology, Branch Bioanalytics and Bioprocesses IZI-BBWe are developing coatings that allow a gentle cell culture. The functional properties of the polymer coatings allow an effective but simple control of cell adhesion. The cells adhere and proliferate on our surfaces as on standard cell substrates at the usual cultivation temperatures. When the temperature decreases by a few degrees, the polymer surfaces become cell-repellent. As a result, the cells reduce the surface contact and can be detached from the surface by light rinsing.
Immunfluoreszenzfärbung zum Nachweis der Stammzelldifferenzierung zu Herzmuskelzellen.
Immunofluorescence staining for the detection of stem cell differentiation in heart muscle cells
© Fraunhofer IZI-BB
Immunfluoreszenzfärbung zum Nachweis von Stammzellmarkern.
Immunofluorescence staining for the detection of stem cell markers
© Fraunhofer IZI-BB
Physiological and functional surfaces for cell cultivation
Adherent cells on a surface coated with a pattern of thermoresponsive polymers.
Three-dimensional image of a fibroblast cell: Confocal microscopy of an immunofluorescence staining to examine proteins relevant to cell adhesion.
Services
- Improvement of the biocompatibility of synthetic surfaces by coating with polymers and biomolecules
- Thermoresponsive polymer coating for controlling cell adhesion
- Various coating methods: spin coating, spray coating, spotting, printing
- Embedding of cells in three-dimensional matrices
- Spheroid culture of cells
- Production and adaptation of microfluidic systems for physiological and controlled cell cultivation