Fraunhofer Institute for Cell Therapy and Immunology, Branch Bioanalytics and Bioprocesses IZI-BBCell Line Development for Customized in vitro Models
The development of cell lines is crucial for biomedical research, drug development, and basic research, as it ensures consistency and reproducibility of results. We develop customized 2D/3D cell models for specialized applications and assays. For genetic modification, such as the integration of Genes of Interest (GOIs), we use transient transfection, stable genomic transfection via viral methods, or CRISPR/Cas. Our innovative recombinant expression systems enable targeted expression of inducible genes or proteins. As starting material, we use primary cells, primary-like cells, and cell lines, including human primary cells and liver tumor cell lines, as well as high-performance serum-free cultured CHO suspension cells. For specialized applications, we have successfully developed cell lines with recombinant expression of human phase I enzymes (CYPs) or GPCRs, making a significant contribution to drug research.
Characteristics and Benefit of the Technology
- High flexibility through cell line adaptation to different culture conditions
- Defined serum-free cultivation
- Physiological 3D cell models with high significance
- Suspension cultivation for high cell yields
- Coculture models for cell-cell interaction analyses
- Access to physiologically critical targets such as “difficult-to-express proteins” through inducible recombinant expression
